Under the influence of any of these factors is necessary to facilitate fertilization injecting sperm into the egg. This is done using microscopy equipment, equipped with systems that allow injection micromanipulation. If fertilization occurs and embryos develop, they are transferred to the uterus in order to continue multiplication and development, to acquire the ability to implant and create a pregnancy.

The stages of ICSI are similar to IVF, the difference is how the eggs are fertilized. Thus sperm are trained for injection and the embryologist according to criteria and selection techniques determines which sperm is used to inject it into the egg. Success rates with ICSI are greatly influenced by the quality of sperm preparation and skill in micromanipulation. The ICSI, combined with IVF is the most effective treatment for male infertility, with a success rate in our institute from 50% to 65% in treatments to generate a pregnancy.